验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, K-562 cells, mouse lung tissue, mouse thymus tissue, rat brain tissue |
| Positive IHC detected in | human cervical cancer tissue, human lung tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12816-1-AP targets ITPKB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | ITPKB fusion protein Ag3456 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | inositol 1,4,5-trisphosphate 3-kinase B |
| Synonyms | InsP 3 kinase B, IP3 3 kinase B, IP3K, IP3K B, IP3KB, ITPKB, PIG37 |
| Calculated Molecular Weight | 946 aa, 102 kDa |
| Observed Molecular Weight | 102-110 kDa |
| GenBank Accession Number | BC015009 |
| Gene Symbol | ITPKB |
| Gene ID (NCBI) | 3707 |
| RRID | AB_2129846 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27987 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Inositol 1,4,5-trisphosphate 3-kinase isoform B (IITPKB) is also named as IP3KB and possesses important roles in the development of immune cells. IP3KB can be targeted to multiple cellular compartments, among them nuclear localization and binding in close proximity to the plasma membrane. The B isoform is the only IP3K that is almost ubiquitously expressed in mammalian cells(PMID:21148483).Three isoforms of IP3KB occur in mammals, which are distinguished by different molecular masses, sensitivity to Ca2+/CaM(calmodulin), intracellular distribution and tissue expression (PMID:16173920). ITPKB can be cleaved into many fragments, this antibody can recognize the N-terminal peptide fragment.(PMID:15130091)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ITPKB antibody 12816-1-AP | Download protocol |
| IHC protocol for ITPKB antibody 12816-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis
| ||
Cell Systematic discovery of mutation-directed neo-protein-protein interactions in cancer. | ||
J Allergy Clin Immunol Severe combined immunodeficiency caused by inositol-trisphosphate 3-kinase B (ITPKB) deficiency. | ||
EMBO Mol Med miR-132 loss de-represses ITPKB and aggravates amyloid and TAU pathology in Alzheimer's brain.
| ||
Sci Signal Inositol 1,4,5-trisphosphate 3-kinase B promotes Ca2+ mobilization and the inflammatory activity of dendritic cells. | ||
Front Mol Biosci Novel cuproptosis metabolism-related molecular clusters and diagnostic signature for Alzheimer's disease |
