验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, K-562 cells, mouse lung tissue, mouse thymus tissue, rat brain tissue |
| Positive IHC detected in | human cervical cancer tissue, human lung tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12816-1-AP targets ITPKB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ITPKB fusion protein Ag3456 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | inositol 1,4,5-trisphosphate 3-kinase B |
| 别名 | InsP 3 kinase B, IP3 3 kinase B, IP3K, IP3K B, IP3KB, ITPKB, PIG37 |
| 计算分子量 | 946 aa, 102 kDa |
| 观测分子量 | 102-110 kDa |
| GenBank蛋白编号 | BC015009 |
| 基因名称 | ITPKB |
| Gene ID (NCBI) | 3707 |
| RRID | AB_2129846 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P27987 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Inositol 1,4,5-trisphosphate 3-kinase isoform B (IITPKB) is also named as IP3KB and possesses important roles in the development of immune cells. IP3KB can be targeted to multiple cellular compartments, among them nuclear localization and binding in close proximity to the plasma membrane. The B isoform is the only IP3K that is almost ubiquitously expressed in mammalian cells(PMID:21148483).Three isoforms of IP3KB occur in mammals, which are distinguished by different molecular masses, sensitivity to Ca2+/CaM(calmodulin), intracellular distribution and tissue expression (PMID:16173920). ITPKB can be cleaved into many fragments, this antibody can recognize the N-terminal peptide fragment.(PMID:15130091)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ITPKB antibody 12816-1-AP | Download protocol |
| IHC protocol for ITPKB antibody 12816-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis
| ||
Cell Systematic discovery of mutation-directed neo-protein-protein interactions in cancer. | ||
J Allergy Clin Immunol Severe combined immunodeficiency caused by inositol-trisphosphate 3-kinase B (ITPKB) deficiency. | ||
EMBO Mol Med miR-132 loss de-represses ITPKB and aggravates amyloid and TAU pathology in Alzheimer's brain.
| ||
Sci Signal Inositol 1,4,5-trisphosphate 3-kinase B promotes Ca2+ mobilization and the inflammatory activity of dendritic cells. | ||
Front Mol Biosci Novel cuproptosis metabolism-related molecular clusters and diagnostic signature for Alzheimer's disease |
