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IVD Monoclonal antibody, PBS Only

IVD Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D12A5

Cat no : 66032-1-PBS

Print datasheet

Synonyms

Isovaleryl-CoA dehydrogenase, mitochondrial, EC:1.3.8.4, EC:1.3.8.1, Butyryl-CoA dehydrogenase, ACAD2

验证数据展示

  • WB analysis using 66032-1-Ig (same clone as 66032-1-PBS)

    WB analysis using 66032-1-Ig (same clone as 66032-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    IF Staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    IF Staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66032-1-Ig (same clone as 66032-1-PBS)

    IF Staining of MCF-7 using 66032-1-Ig (same clone as 66032-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using IVD antibody 66032-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

产品信息

66032-1-PBS targets IVD in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen IVD fusion protein Ag18042 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name isovaleryl Coenzyme A dehydrogenase
Synonyms Isovaleryl-CoA dehydrogenase, mitochondrial, EC:1.3.8.4, EC:1.3.8.1, Butyryl-CoA dehydrogenase, ACAD2
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 41-46 kDa
GenBank Accession NumberBC017202
Gene Symbol IVD
Gene ID (NCBI) 3712
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26440
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.