验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, NIH/3T3 cells |
| Positive IHC detected in | human skin cancer tissue, human thymus tissue, human oesophagus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55328-1-AP targets Involucrin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | involucrin |
| 别名 | involucrin, IVL |
| 计算分子量 | 69 kDa |
| 观测分子量 | 80-90 kDa, 56-70 kDa |
| GenBank蛋白编号 | NM_005547 |
| 基因名称 | Involucrin |
| Gene ID (NCBI) | 3713 |
| RRID | AB_11182270 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07476 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Involucrin antibody 55328-1-AP | Download protocol |
| IHC protocol for Involucrin antibody 55328-1-AP | Download protocol |
| IF protocol for Involucrin antibody 55328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis. | ||
J Ethnopharmacol The Water Extracts from the Oil Cakes of Prinsepia utilis Repair the Epidermal Barrier via Up-Regulating Corneocyte Envelope-Proteins, Lipid Synthases, and Tight Junction Proteins | ||
J Eur Acad Dermatol Venereol Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi. | ||
Oncotarget Transcriptional suppression of microRNA-27a contributes to laryngeal cancer differentiation via GSK-3β-involved Wnt/β-catenin pathway. | ||
Front Oncol Cystatin-B Negatively Regulates the Malignant Characteristics of Oral Squamous Cell Carcinoma Possibly Via the Epithelium Proliferation/Differentiation Program | ||
Int J Mol Sci Water-Soluble Fullerenol C60(OH)36 toward Effective Anti-Air Pollution Induced by Urban Particulate Matter in HaCaT Cell. |
