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IVNS1ABP Polyclonal antibody

IVNS1ABP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14741-1-AP

Print datasheet

Synonyms

ARA3, FLARA3, HSPC068, IVNS1ABP, KIAA0850, ND1, NS 1, NS1, NS1 binding protein, NS1 BP, NS1BP

验证数据展示

  • WB analysis of mouse heart using 14741-1-AP

    WB analysis of mouse heart using 14741-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 14741-1-AP

    IHC staining of human kidney using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14741-1-AP (IVNS1ABP antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 14741-1-AP

    IHC staining of human kidney using 14741-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14741-1-AP (IVNS1ABP antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse heart using 14741-1-AP

    IP experiment of mouse heart using 14741-1-AP

    IP result of anti-IVNS1ABP (IP:14741-1-AP, 4ug; Detection:14741-1-AP 1:800) with mouse heart tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse heart tissue, Apoptosised HeLa cells, HEK-293 cells, HeLa cells, K-562 cells, mouse ovary tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14741-1-AP targets IVNS1ABP in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen IVNS1ABP fusion protein Ag6412 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name influenza virus NS1A binding protein
Synonyms ARA3, FLARA3, HSPC068, IVNS1ABP, KIAA0850, ND1, NS 1, NS1, NS1 binding protein, NS1 BP, NS1BP
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC067739
Gene Symbol IVNS1ABP
Gene ID (NCBI) 10625
RRIDAB_10638784
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6Y0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IVNS1ABP antibody 14741-1-APDownload protocol
IHC protocol for IVNS1ABP antibody 14741-1-APDownload protocol
IP protocol for IVNS1ABP antibody 14741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Global landscape of HIV-human protein complexes.

Authors - Stefanie Jäger
View Article