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验证数据展示

  • WB analysis using 30324-1-AP

    WB analysis using 30324-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30324-1-AP (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 30324-1-AP

    IHC staining of mouse liver using 30324-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 30324-1-AP

    IHC staining of mouse liver using 30324-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 30324-1-AP

    IF Staining of mouse liver using 30324-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Icam-1 antibody (30324-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ICAM-1/CD54 Polyclonal antibody

ICAM-1/CD54 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 30324-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Icam-1, CD54, MALA-2, Icam1, ICAM 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, rat liver tissue, mouse liver tissue, mouse spleen tissue
Positive IHC detected inmouse liver tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30324-1-AP targets ICAM-1/CD54 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intercellular adhesion molecule 1
别名 Icam-1, CD54, MALA-2, Icam1, ICAM 1
计算分子量59 kDa
观测分子量 95-110 kDa
GenBank蛋白编号NM_010493
基因名称 ICAM-1
Gene ID (NCBI) 15894
RRIDAB_3669710
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13597
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ICAM-1 (Intercellular Adhesion Molecule-1, CD54) is a glycosylated, transmembrane protein, a member of Ig super family. This protein involves in cell adhesion by interacting with a number of 2 integrin family members such as LFA-1 and Mac-1. Its expression is regulated by chemokines. This antibody is raised against mouse Icam-1.

实验方案

Product Specific Protocols
WB protocol for ICAM-1/CD54 antibody 30324-1-APDownload protocol
IHC protocol for ICAM-1/CD54 antibody 30324-1-APDownload protocol
IF protocol for ICAM-1/CD54 antibody 30324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols