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ICAM-1/CD54 Polyclonal antibody

ICAM-1/CD54 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30324-1-AP

Print datasheet

Synonyms

Icam-1, CD54, MALA-2, Icam1, ICAM 1

验证数据展示

  • WB analysis using 30324-1-AP

    WB analysis using 30324-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30324-1-AP (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 30324-1-AP

    IHC staining of mouse liver using 30324-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 30324-1-AP

    IHC staining of mouse liver using 30324-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30324-1-AP (Icam-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 30324-1-AP

    IF Staining of mouse liver using 30324-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Icam-1 antibody (30324-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, rat liver tissue, mouse liver tissue, mouse spleen tissue
Positive IHC detected inmouse liver tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30324-1-AP targets ICAM-1/CD54 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms Icam-1, CD54, MALA-2, Icam1, ICAM 1
Calculated Molecular Weight59 kDa
Observed Molecular Weight 95-110 kDa
GenBank Accession NumberNM_010493
Gene Symbol ICAM-1
Gene ID (NCBI) 15894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ICAM-1 (Intercellular Adhesion Molecule-1, CD54) is a glycosylated, transmembrane protein, a member of Ig super family. This protein involves in cell adhesion by interacting with a number of 2 integrin family members such as LFA-1 and Mac-1. Its expression is regulated by chemokines. This antibody is raised against mouse Icam-1.

实验方案

Product Specific Protocols
WB protocol for ICAM-1/CD54 antibody 30324-1-APDownload protocol
IHC protocol for ICAM-1/CD54 antibody 30324-1-APDownload protocol
IF protocol for ICAM-1/CD54 antibody 30324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols