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验证数据展示

  • WB analysis of HEK-293 using 25964-1-AP

    WB analysis of HEK-293 using 25964-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25964-1-AP (Icln antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 25964-1-AP

    IF Staining of HEK-293 using 25964-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25964-1-AP (Icln antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Icln Polyclonal antibody

Icln Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25964-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine

Applications

WB, IF/ICC, ELISA

Synonyms

CLCI, CLNS1A, CLNS1B, I(Cln), ICln, Methylosome subunit pICln, pICln, Reticulocyte pICln

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, MDCK cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CLNS1A Ready-To-Use IHC Kit. CLNS1A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25964-1-AP targets Icln in WB, IF/ICC, ELISA applications and shows reactivity with human, Canine samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, Canine
Immunogen Icln fusion protein Ag22923 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chloride channel, nucleotide-sensitive, 1A
Synonyms CLCI, CLNS1A, CLNS1B, I(Cln), ICln, Methylosome subunit pICln, pICln, Reticulocyte pICln
Calculated Molecular Weight 237 aa, 26 kDa
Observed Molecular Weight 36-42 kDa
GenBank Accession NumberBC119634
Gene Symbol CLNS1A
Gene ID (NCBI) 1207
RRIDAB_2880312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54105
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Icln antibody 25964-1-APDownload protocol
IF protocol for Icln antibody 25964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols