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验证数据展示

  • IHC staining of human tonsillitis using 20758-1-AP

    IHC staining of human tonsillitis using 20758-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG lambda chain antibody included
  • IF Staining of human tonsillitis using 20758-1-AP

    IF Staining of human tonsillitis using 20758-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG Lambda Light Chain antibody (20758-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human plasma using 20758-1-AP

    WB analysis of human plasma using 20758-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Human IgG Lambda Light Chain Polyclonal antibody

Human IgG Lambda Light Chain Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 20758-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA and More (1)

别名

IgG Lambda Light Chain, Human IgG lambda chain, IGLC1, Immunoglobulin lambda constant 1, IGLC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy Human IgG lambda chain Ready-To-Use IHC Kit. Human IgG lambda chain primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20758-1-AP targets Human IgG Lambda Light Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human
免疫原 Human IgG Lambda Light Chain fusion protein Ag14693 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin lambda constant 1 (Mcg marker)
别名 IgG Lambda Light Chain, Human IgG lambda chain, IGLC1, Immunoglobulin lambda constant 1, IGLC
计算分子量 233 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC007782
基因名称 IgG Lambda Light Chain
Gene ID (NCBI) 3537
RRIDAB_2878734
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the lambda light chain of human IgG.

实验方案

Product Specific Protocols
WB protocol for Human IgG Lambda Light Chain antibody 20758-1-APDownload protocol
IHC protocol for Human IgG Lambda Light Chain antibody 20758-1-APDownload protocol
IF protocol for Human IgG Lambda Light Chain antibody 20758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC,IF

iScience

SFTSV infection is associated with transient overproliferation of monoclonal lambda-type plasma cells

Authors - Chuansong Quan
View Article