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Human IgG Lambda Light Chain Polyclonal antibody

Human IgG Lambda Light Chain Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20758-1-AP

Print datasheet

Synonyms

IgG Lambda Light Chain, Human IgG lambda chain, IGLC1, Immunoglobulin lambda constant 1, IGLC

验证数据展示

  • IHC staining of human tonsillitis using 20758-1-AP

    IHC staining of human tonsillitis using 20758-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG lambda chain antibody included
  • IF Staining of human tonsillitis using 20758-1-AP

    IF Staining of human tonsillitis using 20758-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG Lambda Light Chain antibody (20758-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human plasma using 20758-1-AP

    WB analysis of human plasma using 20758-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 20758-1-AP (Human IgG Lambda Light Chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy Human IgG lambda chain Ready-To-Use IHC Kit. Human IgG lambda chain primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20758-1-AP targets Human IgG Lambda Light Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human
Immunogen Human IgG Lambda Light Chain fusion protein Ag14693 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin lambda constant 1 (Mcg marker)
Synonyms IgG Lambda Light Chain, Human IgG lambda chain, IGLC1, Immunoglobulin lambda constant 1, IGLC
Calculated Molecular Weight 233 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC007782
Gene Symbol IgG Lambda Light Chain
Gene ID (NCBI) 3537
RRIDAB_2878734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the lambda light chain of human IgG.

实验方案

Product Specific Protocols
WB protocol for Human IgG Lambda Light Chain antibody 20758-1-APDownload protocol
IHC protocol for Human IgG Lambda Light Chain antibody 20758-1-APDownload protocol
IF protocol for Human IgG Lambda Light Chain antibody 20758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC,IF

iScience

SFTSV infection is associated with transient overproliferation of monoclonal lambda-type plasma cells

Authors - Chuansong Quan
View Article