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验证数据展示

  • WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

    IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

    IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • WB analysis of HEK-293 using 66418-1-Ig (same clone as 66418-1-PBS)

    WB analysis of HEK-293 using 66418-1-Ig (same clone as 66418-1-PBS)

    WB result of IkB Alpha antibody (66418-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IkB Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51027-2

    Cytometric bead array standard curve of MP51027-2

    Cytometric bead array standard curve of MP51027-2, NFKBIA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66418-1-PBS. Detection antibody: 60712-1-PBS. Standard:Ag0345. Range: 0.391-100 ng/mL.

  • Featured Product
  • KD/KO验证

IkB Alpha Monoclonal antibody, PBS Only (Capture)

IkB Alpha Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66418-1-PBS

产品说明书

CloneNo. 2G6H7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

IKBA, IκB α, NFKBIA, IkB-alpha, I-kappa-B-alpha

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

产品信息

66418-1-PBS targets IkB Alpha as part of a matched antibody pair:

MP51027-2: 66418-1-PBS capture and 60712-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IkB Alpha fusion protein Ag22040 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
别名 IKBA, IκB α, NFKBIA, IkB-alpha, I-kappa-B-alpha
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004983
基因名称 IkB Alpha
Gene ID (NCBI) 4792
RRIDAB_2881790
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP25963
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.