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  • Featured Product
  • KD/KO Validated

IkB Alpha Monoclonal antibody, PBS Only

IkB Alpha Monoclonal Antibody for WB,Indirect ELISA,IHC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

2G6H7

Cat No : 66418-1-PBS

Print datasheet

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

验证数据展示

  • WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

    IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

    IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • WB analysis of HEK-293 using 66418-1-Ig (same clone as 66418-1-PBS)

    WB analysis of HEK-293 using 66418-1-Ig (same clone as 66418-1-PBS)

    WB result of IkB Alpha antibody (66418-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IkB Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

产品信息

66418-1-PBS targets IkB Alpha in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC Application Description
Tested Reactivity human, mouse, rat
Immunogen IkB Alpha fusion protein Ag22040 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Synonyms I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC004983
Gene Symbol NFKBIA
Gene ID (NCBI) 4792
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP25963
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.