验证数据展示
经过测试的应用
| Positive WB detected in | LPS and Brefeldin A treated RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
29530-1-AP targets IL-1 beta in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | fusion protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 1 beta |
| 别名 | IL 1 beta, IL-1 beta, Il1b, interleukin 1 beta, Interleukin-1 beta |
| 计算分子量 | 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | NM_031512 |
| 基因名称 | IL-1 beta |
| Gene ID (NCBI) | 24494 |
| RRID | AB_2918322 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q63264 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-1 beta antibody 29530-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ann Med Astragaloside IV ameliorate acute alcohol-induced liver injury in mice via modulating gut microbiota and regulating NLRP3/caspase-1 signaling pathway | ||
Front Pharmacol Dapagliflozin promotes angiogenesis in hindlimb ischemia mice by inducing M2 macrophage polarization | ||
Biochim Biophys Acta Mol Basis Dis Farnesoid X receptor overexpression prevents hepatic steatosis through inhibiting AIM2 inflammasome activation in nonalcoholic fatty liver disease | ||
iScience The mechanisms and therapeutic potential of clopidogrel in mitigating diabetic cardiomyopathy in db/db mice | ||
Clin Nutr Untargeted metabolomics and mendelian randomization analysis identify alpha-linolenic acid and linoleic acid as novel biomarkers of perioperative neurocognitive dysfunction | ||
Int Immunopharmacol Adipose-derived stem cell exosomes loaded with icariin attenuated M1 polarization of macrophages via inhibiting the TLR4/Myd88/NF-κB signaling pathway |