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Mouse Il10 Recombinant antibody, PBS Only (Capture)

Il10 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240430A11

Cat no : 82191-5-PBS

Print datasheet

Synonyms

IL-10,IL10, IL-10, interleukin 10, Il 10, CSIF

验证数据展示

  • Sandwich ELISA standard curve of MP00507-4

    Sandwich ELISA standard curve of MP00507-4

    Sandwich ELISA standard curve of MP00507-4, IL-10 Recombinant Matched Antibody Pair - PBS only. 82191-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0026.82191-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

  • Sample test of MP00507-4

    Sample test of MP00507-4

    Mouse splenocytes (1 x 10^cells/mL) were cultured for 4 days in RPMI supplemented with 10% fetal calf serum, 100 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of LPS. The mean IL-10 concentration was determined to be 7.59 pg/mL in unstimulated spleen cell culture supernatant, 2,461.0 pg/mL in IFN-γ and LPS stimulated spleen cell culture supernatant. Mouse splenocytes (1 x 10^cells/mL) were cultured for 3 days in RPMI supplemented with 10% fetal calf serum,1 μg/mL of LPS. The mean IL-10 concentration was determined to be 11.30 pg/mL in unstimulated spleen cell culture supernatant, 143.99 pg/mL in LPS stimulated spleen cell culture supernatant.

  • Cytometric bead array standard curve of MP00507-1

    Cytometric bead array standard curve of MP00507-1

    Cytometric bead array standard curve of MP00507-1, MOUSE IL-10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82191-5-PBS. Detection antibody: 82191-2-PBS. Standard: Eg0026. Range: 0.625-80 ng/mL.

产品信息

82191-5-PBS targets Il10 as part of a matched antibody pair:

MP00507-1: 82191-5-PBS capture and 82191-2-PBS detection (validated in Cytometric bead array)

MP00507-4: 82191-5-PBS capture and 82191-7-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 10
Synonyms IL-10,IL10, IL-10, interleukin 10, Il 10, CSIF
Calculated Molecular Weight 21 kDa
GenBank Accession NumberNM_010548
Gene Symbol Il10
Gene ID (NCBI) 16153
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18893
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C