IL-2 Polyclonal antibody

IL-2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26156-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA and More (2)

Il 2, Il2, Il-2, interleukin 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPMA, Ionomycin and Brefeldin A treated EL-4 cells
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26156-1-AP targets IL-2 in WB, IHC, IF, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, Cell treatment
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 IL-2 fusion protein Ag24079 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 2
别名 Il 2, Il2, Il-2, interleukin 2
计算分子量 19 kDa
GenBank蛋白编号NM-008366
基因名称 IL-2
Gene ID (NCBI) 16183
RRIDAB_2880407
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the calculated molecular weight of IL2?

19 kDa

Where is IL-2 produced?

IL-2 is primarily produced and secreted by CD4+ helper T cells in secondary lymphoid organs. CD8+ T cells, some B cells, and dendritic cells also produce IL-2.

What allows the protein to bind to its receptor?

The N-terminal 20 amino acids are essential for protein-receptor interaction.

What are the post-translational modifications of IL-2?

IL-2 may present glycosylation, yet the function of this modification has not been confirmed. 

What is the role of IL-2?

IL-2 is a key immunoregulatory cytokine that promotes proliferation of helper T cells, upregulating its own secretion, while also increasing activity of regulatory T cells and providing negative feedback to its production and the immune response as a whole. IL-2 is also a growth factor for natural killer cells and an upregulator of B cell antibody expression and secretion. 

What activates the IL-2 pathway?

Combined T cell antigen receptor and CD28 signals allow for maximum activation of the IL-2 pathway. 

How does IL-2 expression change during an immune response?

During an immune response, CD4+ and CD8+ T cells will secrete increased amounts of IL-2 to be consumed by regulatory T cells. 

How do the responses to strong and weak IL-2 signals differ?

Strong IL-2 levels promote antigen-specific T cells to differentiate into effector T cells, responding immediately to a stimulus or threat. Weak IL-2 signals, however, result in effector T cells to differentiate into helper or memory T cells. 

What negatively regulates IL-2?

Immunoregulatory CD4+CD5+ T cells in inhibit IL-2 production by helper T cells.

 

(PMIDs: 22343569, 24376444, 15473953)

实验方案

Product Specific Protocols
WB protocol for IL-2 antibody 26156-1-APDownload protocol
IHC protocol for IL-2 antibody 26156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
human

Invest New Drugs

A novel tetravalent bispecific antibody targeting programmed death 1 and tyrosine-protein kinase Met for treatment of gastric cancer.

Authors - Weihua Hou
humanIHC

J Cancer

IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro.

Authors - Jia-Qian Hu
humanWB

Peptides

Synthesis of potent antagonists of receptors for growth hormone-releasing hormone with antitumor and anti-inflammatory activity.

Authors - Renzhi Cai
mouseWB

Lipids Health Dis

Arachidonic acid induces macrophage cell cycle arrest through the JNK signaling pathway.

Authors - Ziying Shen
humanWB

Lung

Anti-inflammatory Properties of the Alpha-Melanocyte-Stimulating Hormone in Models of Granulomatous Inflammation.

Authors - Abdolrazagh Hashemi Shahraki
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