IL-2 Polyclonal antibody

IL-2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 26156-1-AP

Print datasheet

Synonyms

Il 2, Il2, Il-2, interleukin 2



经过测试的应用

Positive WB detected inPMA, Ionomycin and Brefeldin A treated EL-4 cells
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26156-1-AP targets IL-2 in WB, IHC, IF, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, Cell treatment
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen IL-2 fusion protein Ag24079 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 2
Synonyms Il 2, Il2, Il-2, interleukin 2
Calculated Molecular Weight 19 kDa
GenBank Accession NumberNM-008366
Gene Symbol IL-2
Gene ID (NCBI) 16183
RRIDAB_2880407
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the calculated molecular weight of IL2?

19 kDa

Where is IL-2 produced?

IL-2 is primarily produced and secreted by CD4+ helper T cells in secondary lymphoid organs. CD8+ T cells, some B cells, and dendritic cells also produce IL-2.

What allows the protein to bind to its receptor?

The N-terminal 20 amino acids are essential for protein-receptor interaction.

What are the post-translational modifications of IL-2?

IL-2 may present glycosylation, yet the function of this modification has not been confirmed. 

What is the role of IL-2?

IL-2 is a key immunoregulatory cytokine that promotes proliferation of helper T cells, upregulating its own secretion, while also increasing activity of regulatory T cells and providing negative feedback to its production and the immune response as a whole. IL-2 is also a growth factor for natural killer cells and an upregulator of B cell antibody expression and secretion. 

What activates the IL-2 pathway?

Combined T cell antigen receptor and CD28 signals allow for maximum activation of the IL-2 pathway. 

How does IL-2 expression change during an immune response?

During an immune response, CD4+ and CD8+ T cells will secrete increased amounts of IL-2 to be consumed by regulatory T cells. 

How do the responses to strong and weak IL-2 signals differ?

Strong IL-2 levels promote antigen-specific T cells to differentiate into effector T cells, responding immediately to a stimulus or threat. Weak IL-2 signals, however, result in effector T cells to differentiate into helper or memory T cells. 

What negatively regulates IL-2?

Immunoregulatory CD4+CD5+ T cells in inhibit IL-2 production by helper T cells.

 

(PMIDs: 22343569, 24376444, 15473953)

实验方案

Product Specific Protocols
WB protocol for IL-2 antibody 26156-1-APDownload protocol
IHC protocol for IL-2 antibody 26156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
human

Invest New Drugs

A novel tetravalent bispecific antibody targeting programmed death 1 and tyrosine-protein kinase Met for treatment of gastric cancer.

Authors - Weihua Hou
humanIHC

J Cancer

IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro.

Authors - Jia-Qian Hu
humanWB

Peptides

Synthesis of potent antagonists of receptors for growth hormone-releasing hormone with antitumor and anti-inflammatory activity.

Authors - Renzhi Cai
mouseWB

Lipids Health Dis

Arachidonic acid induces macrophage cell cycle arrest through the JNK signaling pathway.

Authors - Ziying Shen
humanWB

Lung

Anti-inflammatory Properties of the Alpha-Melanocyte-Stimulating Hormone in Models of Granulomatous Inflammation.

Authors - Abdolrazagh Hashemi Shahraki