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IL3RA/CD123 Polyclonal antibody

IL3RA/CD123 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30012-1-AP

Print datasheet

Synonyms

Il3ra, Sut-1, IL-3R-alpha, IL-3RA, IL-3R subunit alpha

验证数据展示

  • WB analysis using 30012-1-AP

    WB analysis using 30012-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30012-1-AP (Il3ra antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 30012-1-AP

    IF Staining of mouse brain using 30012-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Il3ra antibody (30012-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-12 cells, NIH/3T3 cells
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30012-1-AP targets IL3RA/CD123 in WB, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen IL3RA/CD123 fusion protein Ag32002 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 3 receptor, alpha chain
Synonyms Il3ra, Sut-1, IL-3R-alpha, IL-3RA, IL-3R subunit alpha
Calculated Molecular Weight43 kDa
Observed Molecular Weight43 kDa, 60-70 kDa
GenBank Accession NumberNM_008369
Gene Symbol Il3ra
Gene ID (NCBI) 16188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26952
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-3RA, also named as CD123 and SUT-1, is the alpha subunit of the interleukin 3 (IL-3) receptor, which is part of the type I cytokine receptor superfamily (PMID: 25043189). It can regulate the proliferation, survival, and differentiation of hematopoietic cells. In mouse, there are two classes of high-affinity IL-3 receptors. One contains an IL-3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL-5 and GM-CSF receptors. This antibody is specific for mouse Il3ra.

实验方案

Product Specific Protocols
WB protocol for IL3RA/CD123 antibody 30012-1-APDownload protocol
IF protocol for IL3RA/CD123 antibody 30012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols