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验证数据展示

  • WB analysis using 30012-1-AP

    WB analysis using 30012-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30012-1-AP (Il3ra antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 30012-1-AP

    IF Staining of mouse brain using 30012-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Il3ra antibody (30012-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

IL3RA/CD123 Polyclonal antibody

IL3RA/CD123 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30012-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF-P, ELISA

别名

Il3ra, Sut-1, IL-3R-alpha, IL-3RA, IL-3R subunit alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-12 cells, NIH/3T3 cells
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30012-1-AP targets IL3RA/CD123 in WB, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原 IL3RA/CD123 fusion protein Ag32002 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 3 receptor, alpha chain
别名 Il3ra, Sut-1, IL-3R-alpha, IL-3RA, IL-3R subunit alpha
计算分子量43 kDa
观测分子量43 kDa, 60-70 kDa
GenBank蛋白编号NM_008369
基因名称 Il3ra
Gene ID (NCBI) 16188
RRIDAB_3086209
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP26952
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-3RA, also named as CD123 and SUT-1, is the alpha subunit of the interleukin 3 (IL-3) receptor, which is part of the type I cytokine receptor superfamily (PMID: 25043189). It can regulate the proliferation, survival, and differentiation of hematopoietic cells. In mouse, there are two classes of high-affinity IL-3 receptors. One contains an IL-3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL-5 and GM-CSF receptors. This antibody is specific for mouse Il3ra.

实验方案

Product Specific Protocols
WB protocol for IL3RA/CD123 antibody 30012-1-APDownload protocol
IF protocol for IL3RA/CD123 antibody 30012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols