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验证数据展示

  • WB analysis using 27331-1-AP

    WB analysis using 27331-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27331-1-AP (Inhibin alpha antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 27331-1-AP

    IHC staining of mouse ovary using 27331-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27331-1-AP (Inhibin alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse ovary using 27331-1-AP

    IHC staining of mouse ovary using 27331-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27331-1-AP (Inhibin alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Inhibin alpha Polyclonal antibody

Inhibin alpha Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27331-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

INHA, Inhibin-α, Inhibinα, Inhibin-A, Inhibin α

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse ovary tissue, mouse testis tissue, rat ovary tissue
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27331-1-AP targets Inhibin alpha in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Inhibin alpha fusion protein Ag25999 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inhibin, alpha
Synonyms INHA, Inhibin-α, Inhibinα, Inhibin-A, Inhibin α
Calculated Molecular Weight 40 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC006391
Gene Symbol Inhibin alpha
Gene ID (NCBI) 3623
RRIDAB_3085946
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inhibin, a heterodimeric member of the TGF family, consists of an alpha (α) subunit (coded by INHA) and a beta (β) subunit, which combine to produce either inhibin A (αβA) or inhibin B (αβB). Inhibin complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion.

实验方案

Product Specific Protocols
WB protocol for Inhibin alpha antibody 27331-1-APDownload protocol
IHC protocol for Inhibin alpha antibody 27331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols