验证数据展示
经过测试的应用
Positive WB detected in | rat pancreas tissue |
Positive IHC detected in | rat pancreas tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28609-1-AP targets Ins1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | mouse, rat |
Immunogen | Ins1 fusion protein Ag29989 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | insulin I |
Synonyms | Ins 1, Ins1, Ins2 rs1, insulin I, insulin1 |
Calculated Molecular Weight | 12 kDa |
Observed Molecular Weight | 12 kDa |
GenBank Accession Number | NM_008386 |
Gene Symbol | Ins1 |
Gene ID (NCBI) | 16333 |
RRID | AB_2918180 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P01325 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Ins1 antibody 28609-1-AP | Download protocol |
IHC protocol for Ins1 antibody 28609-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |