验证数据展示
经过测试的应用
Positive WB detected in | rat pancreas tissue |
Positive IHC detected in | rat pancreas tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28609-1-AP targets Ins1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | mouse, rat |
免疫原 | Ins1 fusion protein Ag29989 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | insulin I |
别名 | Ins 1, Ins1, Ins2 rs1, insulin I, insulin1 |
计算分子量 | 12 kDa |
观测分子量 | 12 kDa |
GenBank蛋白编号 | NM_008386 |
基因名称 | Ins1 |
Gene ID (NCBI) | 16333 |
RRID | AB_2918180 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P01325 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.
实验方案
Product Specific Protocols | |
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WB protocol for Ins1 antibody 28609-1-AP | Download protocol |
IHC protocol for Ins1 antibody 28609-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |