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验证数据展示

  • WB analysis using 84883-5-RR

    WB analysis using 84883-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 84883-5-RR

    IF Staining of A549 using 84883-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (84883-5-RR, Clone: 242376D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84883-5-RR

    Affinity and Kinetic Characterization of 84883-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84883-5-RR against Human Integrin Alpha V were performed. The affinity constant is 0.121 nM.

Integrin Alpha V Recombinant antibody

Integrin Alpha V Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84883-5-RR

产品说明书

CloneNo. 242376D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ITGAV, 242376D3, CD51, Integrin alpha-V, Integrin alpha-V heavy chain

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HUVEC cells, MCF-7 cells, human placenta tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84883-5-RR targets Integrin Alpha V in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Integrin Alpha V fusion protein Ag25825 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
别名 ITGAV, 242376D3, CD51, Integrin alpha-V, Integrin alpha-V heavy chain
计算分子量 1048 aa, 116 kDa
观测分子量130 kDa
GenBank蛋白编号BC136442
基因名称 Integrin alpha V
Gene ID (NCBI) 3685
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP06756
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha V, also known as CD51, is a type I transmembrane glycoprotein composed of an extracellular domain, a transmembrane region and cytoplasmic domain (PMID: 2430295; 1690718). It can form heterodimers with one of the five beta integrin subunits (beta 1, 3, 5, 6, 8) that recognize ligands containing the sequence Arg-Gly-Asp, such as vitronectin, fibronectin, and osteopontin (PMID: 37087799). Integrin alpha V mainly plays a role in extracellular matrix-mediated cell adhesion and migration, differentiation, wound healing, inflammation, tumorigenesis, proliferation, angiogenesis, and metastasis.

实验方案

Product Specific Protocols
WB protocol for Integrin Alpha V antibody 84883-5-RRDownload protocol
IF protocol for Integrin Alpha V antibody 84883-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols