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Integrin Beta 4 Monoclonal antibody, PBS Only

Integrin Beta 4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C2A11

Cat no : 66922-2-PBS

Print datasheet

Synonyms

ITGB4, integrin, beta 4, Integrin beta-4, GP150, CD104

验证数据展示

  • WB analysis using 66922-2-Ig (same clone as 66922-2-PBS)

    WB analysis using 66922-2-Ig (same clone as 66922-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 66922-2-Ig (same clone as 66922-2-PBS)

    IHC staining of human skin cancer using 66922-2-Ig (same clone as 66922-2-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ITGB4 antibody included
  • IHC staining of human skin cancer using 66922-2-Ig (same clone as 66922-2-PBS)

    IHC staining of human skin cancer using 66922-2-Ig (same clone as 66922-2-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ITGB4 antibody included
  • IF Staining of A431 using 66922-2-Ig (same clone as 66922-2-PBS)

    IF Staining of A431 using 66922-2-Ig (same clone as 66922-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 4 antibody (66922-2-Ig, Clone: 1C2A11 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66922-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ITGB4 Ready-To-Use IHC Kit. ITGB4 primary antibody included.

产品信息

66922-2-PBS targets Integrin Beta 4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Integrin Beta 4 fusion protein Ag17395 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name integrin, beta 4
Synonyms ITGB4, integrin, beta 4, Integrin beta-4, GP150, CD104
Calculated Molecular Weight 1752 aa, 195 kDa
Observed Molecular Weight 200-210 kDa
GenBank Accession NumberBC126411
Gene Symbol Integrin beta 4
Gene ID (NCBI) 3691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16144
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.