Integrin alpha 1 Polyclonal antibody
Integrin alpha 1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, rat lung tissue |
Positive IHC detected in | human placenta tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human placenta tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
29042-1-AP targets Integrin alpha 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, rat |
Cited Reactivity | human |
Immunogen | Integrin alpha 1 fusion protein Ag30613 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | integrin, alpha 1 |
Synonyms | ITGA1, VLA 1, Laminin and collagen receptor, integrin, alpha 1, CD49a |
Calculated Molecular Weight | 1179 aa, 131 kDa |
Observed Molecular Weight | 180 kDa |
GenBank Accession Number | BC137121 |
Gene Symbol | Integrin alpha 1 |
Gene ID (NCBI) | 3672 |
RRID | AB_2881238 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P56199 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Integrin alpha 1 antibody 29042-1-AP | Download protocol |
IHC protocol for Integrin alpha 1 antibody 29042-1-AP | Download protocol |
IF protocol for Integrin alpha 1 antibody 29042-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Development TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration | ||
PeerJ Production of recombinant human epidermal growth factor fused with HaloTag protein and characterisation of its biological functions |