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验证数据展示

  • WB analysis using 29042-1-AP

    WB analysis using 29042-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 29042-1-AP

    IHC staining of human placenta using 29042-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Integrin alpha-1 antibody included
  • IHC staining of human placenta using 29042-1-AP

    IHC staining of human placenta using 29042-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Integrin alpha-1 antibody included
  • IF Staining of human placenta using 29042-1-AP

    IF Staining of human placenta using 29042-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha-1 antibody (29042-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Integrin alpha 1 Polyclonal antibody

Integrin alpha 1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 29042-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF-P, ELISA

别名

ITGA1, VLA 1, Laminin and collagen receptor, integrin, alpha 1, CD49a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, rat lung tissue
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Integrin alpha-1 Ready-To-Use IHC Kit. Integrin alpha-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

29042-1-AP targets Integrin alpha 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human
免疫原 Integrin alpha 1 fusion protein Ag30613 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrin, alpha 1
别名 ITGA1, VLA 1, Laminin and collagen receptor, integrin, alpha 1, CD49a
计算分子量 1179 aa, 131 kDa
观测分子量180 kDa
GenBank蛋白编号BC137121
基因名称 Integrin alpha 1
Gene ID (NCBI) 3672
RRIDAB_2881238
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56199
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

实验方案

Product Specific Protocols
WB protocol for Integrin alpha 1 antibody 29042-1-APDownload protocol
IHC protocol for Integrin alpha 1 antibody 29042-1-APDownload protocol
IF protocol for Integrin alpha 1 antibody 29042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Development

TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration

Authors - Binbin Li
View Article
humanWB

PeerJ

Production of recombinant human epidermal growth factor fused with HaloTag protein and characterisation of its biological functions

Authors - Mengru Bai
View Article