Integrin alpha 1 Polyclonal antibody

Integrin alpha 1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29042-1-AP

Print datasheet

Synonyms

ITGA1, VLA 1, Laminin and collagen receptor, integrin, alpha 1, CD49a



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, rat lung tissue
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Integrin alpha-1 Ready-To-Use IHC Kit. Integrin alpha-1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

29042-1-AP targets Integrin alpha 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen Integrin alpha 1 fusion protein Ag30613 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, alpha 1
Synonyms ITGA1, VLA 1, Laminin and collagen receptor, integrin, alpha 1, CD49a
Calculated Molecular Weight 1179 aa, 131 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberBC137121
Gene Symbol Integrin alpha 1
Gene ID (NCBI) 3672
RRIDAB_2881238
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56199
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

实验方案

Product Specific Protocols
WB protocol for Integrin alpha 1 antibody 29042-1-APDownload protocol
IHC protocol for Integrin alpha 1 antibody 29042-1-APDownload protocol
IF protocol for Integrin alpha 1 antibody 29042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Development

TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration

Authors - Binbin Li
humanWB

PeerJ

Production of recombinant human epidermal growth factor fused with HaloTag protein and characterisation of its biological functions

Authors - Mengru Bai