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验证数据展示

  • WB analysis using 68561-1-Ig

    WB analysis using 68561-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Integrin alpha-5 Monoclonal antibody

Integrin alpha-5 Monoclonal Antibody for WB, ELISA
Cat No. 68561-1-Ig

Print datasheet

CloneNo. 3B9E5

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, ELISA

Synonyms

CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, rat colon tissue, K-562 cells, Jurkat cells, pig colon tissue, HeLa cells, mouse colon tissue, mouse small intestine tissue
Planning an IHC experiment? We recommend our IHCeasy ITGA5 Ready-To-Use IHC Kit. ITGA5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68561-1-Ig targets Integrin alpha-5 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen Integrin alpha-5 fusion protein Ag33642 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Synonyms CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A
Calculated Molecular Weight 115 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC008786
Gene Symbol Integrin alpha 5
Gene ID (NCBI) 3678
RRIDAB_3085263
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08648
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

实验方案

Product Specific Protocols
WB protocol for Integrin alpha-5 antibody 68561-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols