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验证数据展示

  • WB analysis using 68561-1-Ig

    WB analysis using 68561-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Integrin alpha-5 Monoclonal antibody

Integrin alpha-5 Monoclonal Antibody for WB, ELISA
Cat No. 68561-1-Ig

产品说明书

CloneNo. 3B9E5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, ELISA

别名

ITGA5, 3B9E5, CD49 antigen-like family member E, CD49e, FNRA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, rat colon tissue, HeLa cells, K-562 cells, pig colon tissue, rabbit colon tissue, mouse colon tissue, mouse small intestine tissue
Planning an IHC experiment? We recommend our IHCeasy ITGA5 Ready-To-Use IHC Kit. ITGA5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68561-1-Ig targets Integrin alpha-5 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Integrin alpha-5 fusion protein Ag33642 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
别名 ITGA5, 3B9E5, CD49 antigen-like family member E, CD49e, FNRA
计算分子量 115 kDa
观测分子量140 kDa
GenBank蛋白编号BC008786
基因名称 Integrin alpha 5
Gene ID (NCBI) 3678
RRIDAB_3085263
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08648
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

实验方案

Product Specific Protocols
WB protocol for Integrin alpha-5 antibody 68561-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols