验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, A431 cells, mouse skin tissue, rat lung tissue, HepG2 cells |
| Positive IHC detected in | mouse skin tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27189-1-AP targets Integrin alpha 6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey, bovine, hamster |
| 免疫原 | Integrin alpha 6 fusion protein Ag25835 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | integrin, alpha 6 |
| 别名 | ITGA6, integrin, alpha 6, Integrin alpha-6 light chain, Integrin alpha-6 heavy chain, Integrin alpha-6 |
| 计算分子量 | 1073 aa, 119 kDa |
| 观测分子量 | 110-130 kDa |
| GenBank蛋白编号 | BC136456 |
| 基因名称 | Integrin alpha 6 |
| Gene ID (NCBI) | 3655 |
| RRID | AB_2880792 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23229 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin alpha 6 antibody 27189-1-AP | Download protocol |
| IHC protocol for Integrin alpha 6 antibody 27189-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells | ||
Cells Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak | ||
Virulence Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection. | ||
Stem Cell Res Ther Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth. | ||
J Virol Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus. | ||
Vascul Pharmacol Midkine mediates dysfunction of liver sinusoidal endothelial cells through integrin α4 and α6 |
