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验证数据展示

  • WB analysis using 84463-3-RR

    WB analysis using 84463-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84463-3-RR (Integrin alpha 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 84463-3-RR

    IF Staining of A431 using 84463-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Integrin alpha-6 antibody (84463-3-RR, Clone: 241703B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 84463-3-RR

    Affinity and Kinetic Characterization of 84463-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84463-3-RR against Human Integrin alpha 6 were performed. The affinity constant is 0.614 nM.

Integrin alpha 6 Recombinant antibody

Integrin alpha 6 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84463-3-RR

产品说明书

CloneNo. 241703B5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ITGA6, Integrin alpha-6 light chain, Integrin alpha-6 heavy chain, Integrin alpha-6, CD49f

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84463-3-RR targets Integrin alpha 6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, alpha 6
别名 ITGA6, Integrin alpha-6 light chain, Integrin alpha-6 heavy chain, Integrin alpha-6, CD49f
计算分子量 1073 aa, 119 kDa
观测分子量120 kDa
GenBank蛋白编号BC136456
基因名称 Integrin alpha 6
Gene ID (NCBI) 3655
RRIDAB_3671985
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP23229
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Integrin alpha 6 antibody 84463-3-RRDownload protocol
IF protocol for Integrin alpha 6 antibody 84463-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols