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Integrin alpha-8 Polyclonal antibody

Integrin alpha-8 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30714-1-AP

Print datasheet

Synonyms

Integrin alpha 8, integrin, alpha 8, ITGA8

验证数据展示

  • WB analysis using 30714-1-AP

    WB analysis using 30714-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 30714-1-AP

    IHC staining of mouse lung using 30714-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 30714-1-AP

    IHC staining of mouse lung using 30714-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse lung using 30714-1-AP

    IF Staining of mouse lung using 30714-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Integrin alpha-8 antibody (30714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse lung using 30714-1-AP

    IF Staining of mouse lung using 30714-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Integrin alpha-8 antibody (30714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue, rat spleen tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse lung tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30714-1-AP targets Integrin alpha-8 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Integrin alpha-8 fusion protein Ag33507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, alpha 8
Synonyms Integrin alpha 8, integrin, alpha 8, ITGA8
Calculated Molecular Weight117 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberNM_003638
Gene Symbol Integrin alpha 8
Gene ID (NCBI) 8516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53708
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. Integrin alpha-8 is a single-pass type I membrane protein that forms a heterodimer with integrin beta-1 to serve as a receptor for RGD-containing matrix molecules (fibronectin, vitronectin, tenascin C, osteopontin, and nephronectin) (PMID: 33000355). Integrin alpha-8 beta-1 plays a critical role in epithelial-mesenchymal interactions and functions in the genesis of kidney and probably of other organs (PMID: 9054500; 19769957). The apparent molecular weight of integrin alpha-8 is larger than the calculated molecular weight of 117 kDa, possibly due to glycosylation (PMID: 7768999).

实验方案

Product Specific Protocols
WB protocol for Integrin alpha-8 antibody 30714-1-APDownload protocol
IHC protocol for Integrin alpha-8 antibody 30714-1-APDownload protocol
IF protocol for Integrin alpha-8 antibody 30714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols