验证数据展示
经过测试的应用
Positive WB detected in | mouse lung tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
55068-1-AP targets Integrin alpha-8 in WB, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | integrin, alpha 8 |
别名 | Integrin alpha 8, Integrin alpha-8, integrin, alpha 8, ITGA8 |
计算分子量 | 117 kDa |
观测分子量 | 140 kDa |
GenBank蛋白编号 | NM_003638 |
基因名称 | Integrin alpha 8 |
Gene ID (NCBI) | 8516 |
RRID | AB_3086432 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P53708 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. Integrin alpha-8 is a single-pass type I membrane protein that forms a heterodimer with integrin beta-1 to serve as a receptor for RGD-containing matrix molecules (fibronectin, vitronectin, tenascin C, osteopontin, and nephronectin) (PMID: 33000355). Integrin alpha-8 beta-1 plays a critical role in epithelial-mesenchymal interactions and functions in the genesis of kidney and probably of other organs (PMID: 9054500; 19769957). The apparent molecular weight of integrin alpha-8 is larger than the calculated molecular weight of 117 kDa, possibly due to glycosylation (PMID: 7768999).
实验方案
Product Specific Protocols | |
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WB protocol for Integrin alpha-8 antibody 55068-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |