• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Integrin alpha-9 Polyclonal antibody

Integrin alpha-9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 28348-1-AP

Print datasheet

Synonyms

ALPHA RLC, Integrin alpha 9, Integrin alpha RLC, Integrin alpha-9, integrin, alpha 9, ITGA4L, ITGA9, RLC

验证数据展示

  • WB analysis using 28348-1-AP

    WB analysis using 28348-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28348-1-AP (Integrin alpha 9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28348-1-AP targets Integrin alpha-9 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen Integrin alpha-9 fusion protein Ag28815 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, alpha 9
Synonyms ALPHA RLC, Integrin alpha 9, Integrin alpha RLC, Integrin alpha-9, integrin, alpha 9, ITGA4L, ITGA9, RLC
Calculated Molecular Weight 114 kDa
Observed Molecular Weight135 kDa
GenBank Accession NumberBC030198
Gene Symbol Integrin alpha 9
Gene ID (NCBI) 3680
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13797
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). Integrin alpha 9 contains a large N-terminal extracellular domain with 7 conserved repeats of putative metal-binding domains, a transmembrane segment, and a short C-terminal cytoplasmic tail (PMID: 8245132). Integrin alpha 9 interacts with integrin beta 1 generating α9β1 heterodimer, which is expressed in many cell types including epithelial cells, neutrophils, hepatocytes, muscle and endothelial cells (PMID: 21975548). It binds to a diversity of ligands in the extracellular matrix, like the a-disintegrin and metalloprotease (ADAM) family, THBS1, EMILIN1, VEGF, tenascin-C, osteopontin, fibronectin, and VCAM1 (PMID: 21975548; 33790909). Integrin α9β1 has been implicated in diverse biological functions including angiogenesis, lymphangiogenesis, lymphatic valve morphogenesis, granulopoiesis and tumorigenesis (PMID: 20179422).

实验方案

Product Specific Protocols
WB protocol for Integrin alpha-9 antibody 28348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols