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验证数据展示

  • WB analysis using 83514-3-RR (same clone as 83514-3-PBS)

    WB analysis using 83514-3-RR (same clone as 83514-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00512-4

    Sandwich ELISA standard curve of MP00512-4

    Sandwich ELISA standard curve of MP00512-4, Integrin Beta 5 Recombinant Matched Antibody Pair - PBS only. 83514-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag29783.83514-1-PBS was HRP conjugated as the detection antibody. Range: 9.766-625 pg/mL.

  • Sample test of MP00512-4

    Sample test of MP00512-4

    The mean Integrin Beta 5 concentration was determined to be 275.71 pg/mL in Hela cell extract based on a 2.8 mg/mL extract load and 190.06 pg/mL in MCF-7 cell extract based on a 3.1 mg/mL extract load.

  • IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

    IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

    IHC staining of human ovary cancer using 83514-3-RR (same clone as 83514-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83514-3-RR (same clone as 83514-3-PBS)

    FC experiment of HeLa using 83514-3-RR (same clone as 83514-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Integrin beta-5 Recombinant antibody (83514-3-RR, Clone:240401G11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83514-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00512-1

    Cytometric bead array standard curve of MP00512-1

    Cytometric bead array standard curve of MP00512-1, Integrin Beta 5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83514-2-PBS. Detection antibody: 83514-3-PBS. Standard: Ag29783. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00512-2

    Cytometric bead array standard curve of MP00512-2

    Cytometric bead array standard curve of MP00512-2, Integrin Beta 5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83514-5-PBS. Detection antibody: 83514-3-PBS. Standard: Ag29783. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83514-3-RR

    Affinity and Kinetic Characterization of 83514-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83514-3-RR against Human Integrin beta-5 were performed. The affinity constant is 0.955 nM.

Integrin beta-5 Recombinant antibody, PBS Only (Capture/Detector)

Integrin beta-5 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83514-3-PBS

产品说明书

CloneNo. 240401G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

ITGB5, integrin, beta 5, Integrin beta 5, 240401G11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83514-3-PBS targets Integrin beta-5 as part of a matched antibody pair:

MP00512-1: 83514-2-PBS capture and 83514-3-PBS detection (validated in Cytometric bead array)

MP00512-2: 83514-5-PBS capture and 83514-3-PBS detection (validated in Cytometric bead array)

MP00512-4: 83514-3-PBS capture and 83514-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Integrin beta-5 fusion protein Ag29783 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, beta 5
别名 ITGB5, integrin, beta 5, Integrin beta 5, 240401G11
计算分子量 88 kDa
观测分子量88-100 kDa
GenBank蛋白编号BC006541
基因名称 Integrin beta 5
Gene ID (NCBI) 3693
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP18084
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ITGB5, also known as Integrin beta-5. It is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869). The molecular weight of ITGB5 is 88 kDa, and the glycosylation modification also exists.