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Involucrin Recombinant antibody

Involucrin Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240566D8

Cat no : 83649-5-RR

Print datasheet

Synonyms

IVL, 240566D8

验证数据展示

  • WB analysis using 83649-5-RR

    WB analysis using 83649-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83649-5-RR (Involucrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 83649-5-RR

    IHC staining of human skin using 83649-5-RR

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 83649-5-RR

    IHC staining of human skin cancer using 83649-5-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83649-5-RR

    Affinity and Kinetic Characterization of 83649-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83649-5-RR against Human Involucrin were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inA431 cells, HaCaT cells, HT-29 cells, HT-1080 cells
Positive IHC detected inhuman skin tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83649-5-RR is Involucrin Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Involucrin fusion protein Ag29585 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name involucrin
Synonyms IVL, 240566D8
Calculated Molecular Weight 68 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberNM_005547
Gene Symbol Involucrin
Gene ID (NCBI) 3713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP07476
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502, 12150517)

实验方案

Product Specific Protocols
WB protocol for Involucrin antibody 83649-5-RRDownload protocol
IHC protocol for Involucrin antibody 83649-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols