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验证数据展示

  • WB analysis of mouse spleen using 13846-1-AP

    WB analysis of mouse spleen using 13846-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 13846-1-AP

    IP experiment of Jurkat using 13846-1-AP

    IP result of anti-JAKMIP1 (IP:13846-1-AP, 3ug; Detection:13846-1-AP 1:1000) with Jurkat cells lysate 4000ug.

  • IHC staining of human gliomas using 13846-1-AP

    IHC staining of human gliomas using 13846-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 13846-1-AP

    IHC staining of human gliomas using 13846-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

JAKMIP1 Polyclonal antibody

JAKMIP1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13846-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA and More (1)

别名

MARLIN1, Janus kinase and microtubule-interacting protein 1, janus kinase and microtubule interacting protein 1, JAMIP1, GABABRBP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, HEK-293 cells, Jurkat cells, mouse liver tissue, mouse testis tissue, Y79 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13846-1-AP targets JAKMIP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 JAKMIP1 fusion protein Ag4816 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 janus kinase and microtubule interacting protein 1
别名 MARLIN1, Janus kinase and microtubule-interacting protein 1, janus kinase and microtubule interacting protein 1, JAMIP1, GABABRBP
计算分子量 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC047075
基因名称 JAKMIP1
Gene ID (NCBI) 152789
RRIDAB_2128793
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96N16
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for JAKMIP1 antibody 13846-1-APDownload protocol
IHC protocol for JAKMIP1 antibody 13846-1-APDownload protocol
IP protocol for JAKMIP1 antibody 13846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Neuron

JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.

Authors - Jamee M Berg
  • KO Validated
View Article
humanWB,IHC

Biomed Pharmacother

Overexpression of JAKMIP1 associates with Wnt/beta-catenin pathway activation and promotes cancer cell proliferation in vitro.

Authors - Okai Isaac I
View Article