验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells, MCF-7 cells, MDA-MB-453s cells |
Planning an IHC experiment? We recommend our IHCeasy JMJD5 Ready-To-Use IHC Kit. JMJD5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20999-1-AP targets JMJD5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | JMJD5 fusion protein Ag15115 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | jumonji domain containing 5 |
| 别名 | JMJD5, jumonji domain containing 5, KDM8, Lysine specific demethylase 8 |
| 计算分子量 | 416 aa, 47 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | BC027911 |
| 基因名称 | JMJD5 |
| Gene ID (NCBI) | 79831 |
| RRID | AB_3085630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N371 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
JmjC (Jumonji-C) domain-containing 5 (JMJD5) (also called KDM8) is a 2-oxoglutarate (2-OG) dependent oxygenase of the JmjC subfamily. By shuttling between the nucleus and cytoplasm, JMJD5 could either associate with histones or cytosolic proteins, and thus plays an important role in embryonic development (PMID:22241836;22402282), circadian regulation (PMID:21115819), osteoclastogenesis (PMID:22375008), and stem-cell renewal (PMID:24740926).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for JMJD5 antibody 20999-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |