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验证数据展示

  • WB analysis using 84804-4-RR

    WB analysis using 84804-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84804-4-RR (JMJD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84804-4-RR

    Affinity and Kinetic Characterization of 84804-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84804-4-RR against Human JMJD5 were performed. The affinity constant is 43.3 pM.

JMJD5 Recombinant antibody

JMJD5 Recombinant Antibody for WB, ELISA
Cat No. 84804-4-RR

产品说明书

CloneNo. 242097E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

JMJD 5, JmjC domain-containing protein 5, EC:1.14.11.73, Bifunctional peptidase and arginyl-hydroxylase JMJD5, 3R

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy JMJD5 Ready-To-Use IHC Kit. JMJD5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84804-4-RR targets JMJD5 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 JMJD5 fusion protein Ag15115 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 jumonji domain containing 5
别名 JMJD 5, JmjC domain-containing protein 5, EC:1.14.11.73, Bifunctional peptidase and arginyl-hydroxylase JMJD5, 3R
计算分子量 416 aa, 47 kDa
观测分子量50-60 kDa
GenBank蛋白编号BC027911
基因名称 JMJD5
Gene ID (NCBI) 79831
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8N371
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for JMJD5 antibody 84804-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols