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JNK Polyclonal antibody, PBS Only

JNK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10023-1-PBS

Print datasheet

Synonyms

MAPK8, JNK1A2, JNK1, JNK 2, JNK 1

验证数据展示

  • WB analysis of NIH/3T3 using 10023-1-AP (same clone as 10023-1-PBS)

    WB analysis of NIH/3T3 using 10023-1-AP (same clone as 10023-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10023-1-AP (same clone as 10023-1-PBS)

    IHC staining of human prostate cancer using 10023-1-AP (same clone as 10023-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAPK8/JNK antibody included
  • IHC staining of human prostate cancer using 10023-1-AP (same clone as 10023-1-PBS)

    IHC staining of human prostate cancer using 10023-1-AP (same clone as 10023-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAPK8/JNK antibody included
  • IF Staining of HeLa using 10023-1-AP (same clone as 10023-1-PBS)

    IF Staining of HeLa using 10023-1-AP (same clone as 10023-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JNK antibody (10023-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10023-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MAPK8/JNK Ready-To-Use IHC Kit. MAPK8/JNK primary antibody included.

产品信息

10023-1-PBS targets JNK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen JNK fusion protein Ag0019 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 8
Synonyms MAPK8, JNK1A2, JNK1, JNK 2, JNK 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 40-45 kDa, 50-55 kDa
GenBank Accession NumberNM_138982
Gene Symbol JNK
Gene ID (NCBI) 5599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP45983
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.