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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 51151-1-AP (same clone as 51151-1-PBS)

    WB analysis using 51151-1-AP (same clone as 51151-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 51151-1-AP (same clone as 51151-1-PBS)

    IHC staining of human colon cancer using 51151-1-AP (same clone as 51151-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAPK8/JNK antibody included
  • IF Staining of SH-SY5Y using 51151-1-AP (same clone as 51151-1-PBS)

    IF Staining of SH-SY5Y using 51151-1-AP (same clone as 51151-1-PBS)

    Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 51151-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

JNK Polyclonal antibody, PBS Only

JNK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 51151-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAPK8/JNK Ready-To-Use IHC Kit. MAPK8/JNK primary antibody included.

产品信息

51151-1-PBS targets JNK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 8
Synonyms
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 44-48 kDa, 50-55 kDa
GenBank Accession NumberNM_138982
Gene Symbol JNK
Gene ID (NCBI) 5599
RRIDAB_2140710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP45983
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.