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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 51151-1-AP (same clone as 51151-1-PBS)

    WB analysis using 51151-1-AP (same clone as 51151-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 51151-1-AP (same clone as 51151-1-PBS)

    IHC staining of human colon cancer using 51151-1-AP (same clone as 51151-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAPK8/JNK antibody included
  • IF Staining of SH-SY5Y using 51151-1-AP (same clone as 51151-1-PBS)

    IF Staining of SH-SY5Y using 51151-1-AP (same clone as 51151-1-PBS)

    Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 51151-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

JNK Polyclonal antibody, PBS Only

JNK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 51151-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAPK8/JNK Ready-To-Use IHC Kit. MAPK8/JNK primary antibody included.

产品信息

51151-1-PBS targets JNK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 8
别名
计算分子量 48 kDa
观测分子量 44-48 kDa, 50-55 kDa
GenBank蛋白编号NM_138982
基因名称 JNK
Gene ID (NCBI) 5599
RRIDAB_2140710
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP45983
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.