- Featured Product
- KD/KO Validated
JTV1 Polyclonal antibody
JTV1 Polyclonal Antibody for WB, IP, IF/ICC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF/ICC, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, K-562 cells, NIH/3T3 cells, rat liver tissue, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse cerebellum tissue, human kidney tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy AIMP2/JTV1 Ready-To-Use IHC Kit. AIMP2/JTV1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10424-1-AP targets JTV1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IF/ICC, IHC, ELISA |
| Cited Applications | WB, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | JTV1 fusion protein Ag0675 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | JTV1 gene |
| Synonyms | AIMP2, JTV1, JTV1 gene, P38, PRO0992, Protein JTV 1 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC002853 |
| Gene Symbol | JTV1 |
| Gene ID (NCBI) | 7965 |
| RRID | AB_513880 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13155 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor. This is a rabbit polyclonal antibody raised against full-length of JTV1. There're two isoforms of JTV1 with MW about 27 kDa and 35 kDa,
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for JTV1 antibody 10424-1-AP | Download protocol |
| IHC protocol for JTV1 antibody 10424-1-AP | Download protocol |
| IF protocol for JTV1 antibody 10424-1-AP | Download protocol |
| IP protocol for JTV1 antibody 10424-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Neurosci Parthanatos mediates AIMP2-activated age-dependent dopaminergic neuronal loss. | ||
J Exp Clin Cancer Res RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization | ||
Nat Commun Molecular determinants of selective clearance of protein inclusions by autophagy. | ||
Proc Natl Acad Sci U S A Role for the obesity-related FTO gene in the cellular sensing of amino acids. | ||
Proc Natl Acad Sci U S A Phosphorylation by the c-Abl protein tyrosine kinase inhibits parkin's ubiquitination and protective function. |
