验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, human kidney tissue, human brain tissue, rat colon tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19740-1-AP targets KALRN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kalirin, RhoGEF kinase |
| 别名 | DUET, duo, HAPIP, Kalirin, kalirin, RhoGEF kinase, KALRN, Protein Duo, TRAD |
| 计算分子量 | 340 kDa |
| 观测分子量 | 200 kDa |
| GenBank蛋白编号 | NM_007064 |
| 基因名称 | KALRN |
| Gene ID (NCBI) | 8997 |
| RRID | AB_10638319 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60229 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KALRN, also named as DUET, DUO, HAPIP and TRAD, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. KALRN promotes the exchange of GDP by GTP. KALRN activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. KALRN induces lamellipodia independent of its GEF activity. The antibody can recognize isoform1 and isoform2 of KALRN.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KALRN antibody 19740-1-AP | Download protocol |
| IHC protocol for KALRN antibody 19740-1-AP | Download protocol |
| IF protocol for KALRN antibody 19740-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
FEBS Lett Kalirin mediates Rac1 activation downstream of calcium/calmodulin-dependent protein kinase II to stimulate glucose uptake during muscle contraction. | ||
Biochem Biophys Res Commun CaMKII and Kalirin, a Rac1-GEF, regulate Akt phosphorylation involved in contraction-induced glucose uptake in skeletal muscle cells. | ||
BMC Womens Health Metabolism-associated molecular classification of cervical cancer
| ||
Mol Cell Neurosci Role and mechanism of EphB3 in epileptic seizures and epileptogenesis through Kalirin |
