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验证数据展示

  • WB analysis using 29782-1-AP

    WB analysis using 29782-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29782-1-AP (KANK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 29782-1-AP

    IHC staining of mouse heart using 29782-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29782-1-AP (KANK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KANK1 antibody included
  • IHC staining of mouse heart using 29782-1-AP

    IHC staining of mouse heart using 29782-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29782-1-AP (KANK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KANK1 antibody included
  • IF Staining of mouse kidney using 29782-1-AP

    IF Staining of mouse kidney using 29782-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using KANK1 antibody (29782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KANK1 Polyclonal antibody

KANK1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 29782-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

KN motif and ankyrin repeat domain-containing protein 1, Kidney ankyrin repeat-containing protein, KIAA0172, KANK, Ankyrin repeat domain-containing protein 15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy KANK1 Ready-To-Use IHC Kit. KANK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29782-1-AP targets KANK1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 KANK1 fusion protein Ag31422 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KN motif and ankyrin repeat domains 1
别名 KN motif and ankyrin repeat domain-containing protein 1, Kidney ankyrin repeat-containing protein, KIAA0172, KANK, Ankyrin repeat domain-containing protein 15
计算分子量147KD
观测分子量130-200 kDa
GenBank蛋白编号NM_015158
基因名称 KANK1
Gene ID (NCBI) 23189
RRIDAB_3086160
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14678
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KANK1 (KN motif and ankyrin repeat domain-containing protein 1) is involved in the control of cytoskeleton formation by regulating actin polymerization, inhibiting actin fiber formation and cell migration (PMID: 25961457). It inhibits RhoA activity, the formation of lamellipodia but not of filopodia (PMID: 25961457, PMID: 5961457).It also inhibits fibronectin-mediated cell spreading and regulates Rac signaling pathways (PMID: 25961457). Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions (PMID: 27410476). KANK1 can be detected 130-200 kDa (PMID: 15823577, PMID: 33253712, PMID: 35805114).

实验方案

Product Specific Protocols
WB protocol for KANK1 antibody 29782-1-APDownload protocol
IHC protocol for KANK1 antibody 29782-1-APDownload protocol
IF protocol for KANK1 antibody 29782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols