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KANK2 Polyclonal antibody

KANK2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20546-1-AP

Print datasheet

Synonyms

MXRA3, Matrix-remodeling-associated protein 3, KN motif and ankyrin repeat domain-containing protein 2, KIAA1518, Ankyrin repeat domain-containing protein 25

验证数据展示

  • IHC staining of human colon using 20546-1-AP

    IHC staining of human colon using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon using 20546-1-AP

    IHC staining of human colon using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 20546-1-AP

    IF Staining of HeLa using 20546-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KANK2 antibody (20546-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive IHC detected inhuman colon tissue, human kidney tissue, mouse heart tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

20546-1-AP targets KANK2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KN motif and ankyrin repeat domains 2
Synonyms MXRA3, Matrix-remodeling-associated protein 3, KN motif and ankyrin repeat domain-containing protein 2, KIAA1518, Ankyrin repeat domain-containing protein 25
Calculated Molecular Weight 91 kDa
GenBank Accession NumberNM_001136191
Gene Symbol KANK2
Gene ID (NCBI) 25959
RRIDAB_10694286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ63ZY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KANK2, also named as ANKRD25, KIAA1518, MXRA3 and SIP, is found as a candidate tumor suppressor for renal cell carcinoma. It is an ankyrin-repeat domain-containing protein. KANK2 has an identical role in the formation of actin stress fibers. The antibody is specific to KANK2.

实验方案

Product Specific Protocols
IHC protocol for KANK2 antibody 20546-1-APDownload protocol
IF protocol for KANK2 antibody 20546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Lab Med

Identification of biomarkers associated with pathological tumor staging and their utility in the diagnosis and prognosis of prostate cancer

Authors - Shiquan Xu
View Article