验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells, U2OS cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25533-1-AP targets KANSL3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | KANSL3 fusion protein Ag22117 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA1310 |
| 别名 | KIAA1310, KAT8 regulatory NSL complex subunit 3, PRTD, NSL3, NSL complex protein NSL3 |
| 计算分子量 | 878 aa, 93 kDa |
| 观测分子量 | 105 kDa |
| GenBank蛋白编号 | BC063792 |
| 基因名称 | KANSL3 |
| Gene ID (NCBI) | 55683 |
| RRID | AB_3085802 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P2N6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KANSL3, or KAT8-associated nonspecific lethal complex subunit 3, is a component of the KANSL complex, which is an important chromatin modifier in higher animals. This complex plays a crucial role in regulating housekeeping genes and is highly conserved across species. KANSL3, along with other KANSL complex members, is essential for cell division and has been shown to interact robustly both in vitro and in vivo. KANSL3 has been found to have a dual role in cellular function. In interphase, KANSL3 is chromatin-bound and regulates the expression of housekeeping genes. During mitosis, KANSL3 relocates to the mitotic spindle and is important for cell division. It has been identified as a microtubule minus-end-binding protein, which is a novel function for a chromatin-binding complex. KANSL3's ability to bind directly to microtubule minus ends is significant, as very few proteins are known to perform this function.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KANSL3 antibody 25533-1-AP | Download protocol |
| IHC protocol for KANSL3 antibody 25533-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |