验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, Jurkat cells, human colon tissue, HeLa cells, HepG2 cells |
| Positive IHC detected in | human brown disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14951-1-AP targets KARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, zebrafish |
| 免疫原 | KARS fusion protein Ag6778 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lysyl-tRNA synthetase |
| 别名 | Lysyl-tRNA synthetase, Lysine--tRNA ligase, KIAA0070, KARS1, EC:2.7.7.- |
| 计算分子量 | 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC004132 |
| 基因名称 | KARS |
| Gene ID (NCBI) | 3735 |
| RRID | AB_2128158 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15046 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KARS (lysyl-tRNA synthetase) is a gene that encodes an enzyme critical for protein synthesis, specifically responsible for attaching the amino acid lysine to its corresponding transfer RNA (tRNA) in both the cytoplasm and mitochondria. This enzyme plays a crucial role in the process of aminoacylation, which is essential for accurate protein translation from mRNA sequences. Mutations in the KARS gene have been associated with a variety of neurological and systemic disorders, reflecting the enzyme's importance in cellular function.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KARS antibody 14951-1-AP | Download protocol |
| IHC protocol for KARS antibody 14951-1-AP | Download protocol |
| IF protocol for KARS antibody 14951-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation. | ||
Nucleic Acids Res Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function. | ||
Nucleic Acids Res Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex. | ||
Cell Chem Biol Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer. | ||
Hum Mutat Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis. | ||
Front Cell Infect Microbiol Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection. |
