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验证数据展示

  • WB analysis of HUVEC using 28390-1-AP

    WB analysis of HUVEC using 28390-1-AP

    WB result of KAT2A/GCN5 antibody (28390-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and KAT2A/GCN5 knockout HUVEC cells.

  • WB analysis using 28390-1-AP

    WB analysis using 28390-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28390-1-AP (KAT2A/GCN5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28390-1-AP

    IF Staining of HeLa using 28390-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAT2A/GCN5 antibody (28390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IP experiment of LNCaP using 28390-1-AP

    IP experiment of LNCaP using 28390-1-AP

    IP result of anti-KAT2A/GCN5 (IP:28390-1-AP, 4ug; Detection:28390-1-AP 1:6000) with LNCaP cells lysate 1200 ug.

  • Featured Product
  • KD/KO验证

KAT2A/GCN5 Polyclonal antibody

KAT2A/GCN5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 28390-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

hGCN5, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HUVEC cells, HeLa cells, LNCaP cells, MCF-7 cells
Positive IP detected inLNCaP cells, hTERT-RPE1 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28390-1-AP targets KAT2A/GCN5 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 KAT2A/GCN5 fusion protein Ag29005 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 K(lysine) acetyltransferase 2A
别名 hGCN5, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48
计算分子量 94 kDa
观测分子量94 kDa
GenBank蛋白编号BC032743
基因名称 KAT2A
Gene ID (NCBI) 2648
RRIDAB_3669658
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92830
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.

实验方案

Product Specific Protocols
WB protocol for KAT2A/GCN5 antibody 28390-1-APDownload protocol
IF protocol for KAT2A/GCN5 antibody 28390-1-APDownload protocol
IP protocol for KAT2A/GCN5 antibody 28390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols