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KAT2A/GCN5 Polyclonal antibody

KAT2A/GCN5 Polyclonal Antibody for IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 28390-1-AP

Print datasheet

Synonyms

hGCN5, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48

验证数据展示

  • IF Staining of HeLa using 28390-1-AP

    IF Staining of HeLa using 28390-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAT2A/GCN5 antibody (28390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IP experiment of LNCaP using 28390-1-AP

    IP experiment of LNCaP using 28390-1-AP

    IP result of anti-KAT2A/GCN5 (IP:28390-1-AP, 4ug; Detection:28390-1-AP 1:6000) with LNCaP cells lysate 1200 ug.

经过测试的应用

Positive IP detected inLNCaP cells, hTERT-RPE1 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28390-1-AP targets KAT2A/GCN5 in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen KAT2A/GCN5 fusion protein Ag29005 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name K(lysine) acetyltransferase 2A
Synonyms hGCN5, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48
Calculated Molecular Weight 94 kDa
Observed Molecular Weight94 kDa
GenBank Accession NumberBC032743
Gene Symbol KAT2A
Gene ID (NCBI) 2648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92830
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.

实验方案

Product Specific Protocols
IF protocol for KAT2A/GCN5 antibody 28390-1-APDownload protocol
IP protocol for KAT2A/GCN5 antibody 28390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols