验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IP | See 1 publications below |
产品信息
28770-1-AP targets PCAF in WB, IP, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse |
| 免疫原 | PCAF fusion protein Ag30238 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | K(lysine) acetyltransferase 2B |
| 别名 | CAF, Histone acetylase PCAF, Histone acetyltransferase PCAF, K(lysine) acetyltransferase 2B, KAT2B, Lysine acetyltransferase 2B, P/CAF, P300/CBP associated factor, PCAF |
| 计算分子量 | 93 kDa |
| 观测分子量 | 95-110 kDa |
| GenBank蛋白编号 | BC060823 |
| 基因名称 | PCAF/KAT2B |
| Gene ID (NCBI) | 8850 |
| RRID | AB_2918200 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92831 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCAF antibody 28770-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res The histone lysine acetyltransferase KAT2B inhibits cholangiocarcinoma growth: evidence for interaction with SP1 to regulate NF2-YAP signaling | ||
Cancer Cell Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite | ||
J Proteome Res Integrative Analysis of Lactylome and Proteome of Hypertrophic Scar To Identify Pathways or Proteins Associated with Disease Development | ||
Mol Med Celastrol promotes DNA damage and apoptosis in uterine corpus endometrial carcinoma via promotion of KAT2B-mediated RBPJ acetylation and repression of MCM4 transcription | ||
J Cell Mol Med SIRT2-mediated deacetylation of ACLY promotes the progression of oesophageal squamous cell carcinoma |