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KBTBD2 Polyclonal antibody

KBTBD2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13871-1-AP

Print datasheet

Synonyms

BKLHD1, KBTBD2, KIAA1489

验证数据展示

  • WB analysis of COLO 320 using 13871-1-AP

    WB analysis of COLO 320 using 13871-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13871-1-AP (KBTBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 13871-1-AP

    WB analysis using 13871-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13871-1-AP (KBTBD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCOLO 320 cells, A549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13871-1-AP targets KBTBD2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KBTBD2 fusion protein Ag4823 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch repeat and BTB (POZ) domain containing 2
Synonyms BKLHD1, KBTBD2, KIAA1489
Calculated Molecular Weight 622 aa, 71 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC047107
Gene Symbol KBTBD2
Gene ID (NCBI) 25948
RRIDAB_2296330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IY47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106). A 60-70 kDa band has also been reported(PMID:27708159).

实验方案

Product Specific Protocols
WB protocol for KBTBD2 antibody 13871-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols