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验证数据展示

  • WB analysis of COLO 320 using 13871-1-AP

    WB analysis of COLO 320 using 13871-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13871-1-AP (KBTBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 13871-1-AP

    WB analysis using 13871-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13871-1-AP (KBTBD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

KBTBD2 Polyclonal antibody

KBTBD2 Polyclonal Antibody for WB, ELISA
Cat No. 13871-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

BKLHD1, KBTBD2, KIAA1489

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, A549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13871-1-AP targets KBTBD2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KBTBD2 fusion protein Ag4823 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch repeat and BTB (POZ) domain containing 2
别名 BKLHD1, KBTBD2, KIAA1489
计算分子量 622 aa, 71 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC047107
基因名称 KBTBD2
Gene ID (NCBI) 25948
RRIDAB_2296330
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IY47
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106). A 60-70 kDa band has also been reported(PMID:27708159).

实验方案

Product Specific Protocols
WB protocol for KBTBD2 antibody 13871-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols