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KBTBD5 Polyclonal antibody

KBTBD5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21836-1-AP

Print datasheet

Synonyms

KBTBD5, KLHL40, Sarcosynapsin, SRYP

验证数据展示

  • WB analysis of mouse skeletal muscle using 21836-1-AP

    WB analysis of mouse skeletal muscle using 21836-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21836-1-AP (KBTBD5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 21836-1-AP

    IHC staining of mouse skeletal muscle using 21836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21836-1-AP (KBTBD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 21836-1-AP

    IHC staining of mouse skeletal muscle using 21836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21836-1-AP (KBTBD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21836-1-AP targets KBTBD5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen KBTBD5 fusion protein Ag16430 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch repeat and BTB (POZ) domain containing 5
Synonyms KBTBD5, KLHL40, Sarcosynapsin, SRYP
Calculated Molecular Weight 621 aa, 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC110491
Gene Symbol KBTBD5
Gene ID (NCBI) 131377
RRIDAB_2878923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2TBA0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KBTBD5, also known as KLHL40 and SRYP, contains a BACK domain, a BTB/POZ domain and 5 Kelch repeats. It is expressed predominantly in skeletal muscle. The KLHL40 gene mutation is responsible for a severe and potentially fatal neuromyopathy (PMID: 38397198).

实验方案

Product Specific Protocols
WB protocol for KBTBD5 antibody 21836-1-APDownload protocol
IHC protocol for KBTBD5 antibody 21836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols