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验证数据展示

  • WB analysis using 28724-1-AP

    WB analysis using 28724-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 28724-1-AP (KCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 28724-1-AP

    IHC staining of rat brain using 28724-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC12A5 antibody included
  • IF Staining of mouse cerebellum using 28724-1-AP

    IF Staining of mouse cerebellum using 28724-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5 antibody (28724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 28724-1-AP

    IF Staining of mouse cerebellum using 28724-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5 antibody (28724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

KCC2/SLC12A5 Polyclonal antibody

KCC2/SLC12A5 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 28724-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat and More (1)

应用

WB, IHC, IF-P, ELISA

别名

hKCC2, K Cl cotransporter 2, KCC2, KCC2/SLC12A5, KIAA1176, Neuronal K Cl cotransporter, SLC12A5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy SLC12A5 Ready-To-Use IHC Kit. SLC12A5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28724-1-AP targets KCC2/SLC12A5 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse, Rat
文献引用反应性human
免疫原 KCC2/SLC12A5 fusion protein Ag30203 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 12 (potassium-chloride transporter), member 5
别名 hKCC2, K Cl cotransporter 2, KCC2, KCC2/SLC12A5, KIAA1176, Neuronal K Cl cotransporter, SLC12A5
观测分子量130-150 kDa
GenBank蛋白编号BC132670
基因名称 KCC2
Gene ID (NCBI) 57468
RRIDAB_2918195
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2X9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases.

实验方案

Product Specific Protocols
WB protocol for KCC2/SLC12A5 antibody 28724-1-APDownload protocol
IHC protocol for KCC2/SLC12A5 antibody 28724-1-APDownload protocol
IF protocol for KCC2/SLC12A5 antibody 28724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

The NTE domain of PTENα/β promotes cancer progression by interacting with WDR5 via its SSSRRSS motif

Authors - Xiaolei Huang
View Article