Kv1.2 Polyclonal antibody

Kv1.2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14235-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

HBK5, HK4, HUKIV, KCNA2, KV1.2, MK2, NGK1, RBK2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, multi-cells/tissue, human heart tissue, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KCNA2 Ready-To-Use IHC Kit. KCNA2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

14235-1-AP targets Kv1.2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 Kv1.2 fusion protein Ag5477 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, shaker-related subfamily, member 2
别名 HBK5, HK4, HUKIV, KCNA2, KV1.2, MK2, NGK1, RBK2
计算分子量 499 aa, 57 kDa
观测分子量 66-75 kDa
GenBank蛋白编号BC043564
基因名称 Kv1.2
Gene ID (NCBI) 3737
RRIDAB_2280576
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16389
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Kv1.2 antibody 14235-1-APDownload protocol
IHC protocol for Kv1.2 antibody 14235-1-APDownload protocol
IP protocol for Kv1.2 antibody 14235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Adv Sci (Weinh)

Branched-Chain Amino Acids Deficiency Promotes Diabetic Neuropathic Pain Through Upregulating LAT1 and Inhibiting Kv1.2 Channel

Authors - Ze-Yu Zhou
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