验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse thymus tissue |
| Positive IF-P detected in | Rat brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14079-1-AP targets KCNA3 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF-P, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | KCNA3 fusion protein Ag5204 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | potassium voltage-gated channel, shaker-related subfamily, member 3 |
| Synonyms | Kv1.3, MK3, HUKIII, HPCN3, HLK3 |
| Calculated Molecular Weight | 575 aa, 64 kDa |
| Observed Molecular Weight | 52 kDa, 75 kDa |
| GenBank Accession Number | BC035059 |
| Gene Symbol | KCNA3 |
| Gene ID (NCBI) | 3738 |
| RRID | AB_2878011 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22001 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kv1.3 ( also known as KCNA3, MK3, HGK5, HLK3, PCN3, HPCN3) contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. It plays an essential role in T cell proliferation and activation. Kv1.3 channels are expressed in in various tissues and cell types including brain, vascular smooth muscle cells and leucocytes. Malfunction of Kv1.3 can alter the immune response, elicit neurotoxic effects or impact on cancer growth(PMID: 24305723; 24133455). 14079-1-AP can detect two isoforms of Kv1.3 other than the canonical channel (60 KDa) : A longer isoform (70 KDa) and a truncated isoform (43 KDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KCNA3 antibody 14079-1-AP | Download protocol |
| IF protocol for KCNA3 antibody 14079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology.
| ||
Int Immunopharmacol Clarithromycin attenuates airway epithelial-mesenchymal transition in ovalbumin-induced asthmatic mice through modulation of Kv1.3 channels and PI3K/Akt signaling | ||
Int J Clin Exp Med Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1. |
