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验证数据展示

  • WB analysis of mouse heart using 14697-1-AP

    WB analysis of mouse heart using 14697-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14697-1-AP (KCNAB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 14697-1-AP

    IHC staining of human brain using 14697-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14697-1-AP (KCNAB1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 14697-1-AP

    IHC staining of human brain using 14697-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14697-1-AP (KCNAB1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 14697-1-AP

    IF Staining of HeLa using 14697-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KCNAB1 antibody (14697-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

KCNAB1 Polyclonal antibody

KCNAB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14697-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

K(+) channel subunit beta-1, K(+) channel subunit beta 1, hKvBeta3, hKvb3, EC:1.1.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, Neuro-2a cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:1500
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14697-1-AP targets KCNAB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KCNAB1 fusion protein Ag6339 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, shaker-related subfamily, beta member 1
别名 K(+) channel subunit beta-1, K(+) channel subunit beta 1, hKvBeta3, hKvb3, EC:1.1.1.-
计算分子量 47 kDa
观测分子量 68 kDa
GenBank蛋白编号BC043166
基因名称 KCNAB1
Gene ID (NCBI) 7881
RRIDAB_2131349
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14722
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KCNAB1 antibody 14697-1-APDownload protocol
IHC protocol for KCNAB1 antibody 14697-1-APDownload protocol
IF protocol for KCNAB1 antibody 14697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols