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验证数据展示

  • WB analysis using 25468-1-AP

    WB analysis using 25468-1-AP

    Rat brain tissues (heated at 100 ° C, heated at 37℃) lysates were subjected to SDS PAGE followed by western blot with 25468-1-AP (KCND3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 25468-1-AP

    IHC staining of mouse cerebellum using 25468-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25468-1-AP

    IHC staining of mouse brain using 25468-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KCND3 Polyclonal antibody

KCND3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25468-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

KV4.3, KSHIVB, KCND3S, KCND3L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, 37℃ incubated rat brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25468-1-AP targets KCND3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KCND3 fusion protein Ag22183 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, Shal-related subfamily, member 3
别名 KV4.3, KSHIVB, KCND3S, KCND3L
计算分子量 655 aa, 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC113477
基因名称 KCND3
Gene ID (NCBI) 3752
RRIDAB_3669478
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UK17
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCND3 gene encodes the voltage-gated potassium ion channel subfamily D member 3, a six trans-membrane protein (Kv4.3), involved in the transient outward K+ current. KCND3 gene defect causes both cardiological and neurological syndromes. Kv4.3 contributes to the cardiac transient outward potassium current (Ito1), the main contributing current to the repolarizing phase 1 of the cardiac action potential.

实验方案

Product Specific Protocols
WB protocol for KCND3 antibody 25468-1-APDownload protocol
IHC protocol for KCND3 antibody 25468-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols