验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18289-1-AP targets KCNE4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | potassium voltage-gated channel, Isk-related family, member 4 |
| 别名 | KCNE4, MinK related peptide 3, MIRP3 |
| 计算分子量 | 18 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | NM_080671 |
| 基因名称 | KCNE4 |
| Gene ID (NCBI) | 23704 |
| RRID | AB_2132300 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WWG9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KCNE4, also named as MIRP3 and MGC2035, is a membrane protein belongs to a family of single transmembrane domain proteins known to have dramatic effect on the gating of certain potassium channels. KCNE4 is expressed strongly in heart, skeletal muscle and kidney. Electrophysiological studies show that human KCNE4 modulates the activation of the KCNQ1 channel.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KCNE4 antibody 18289-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Sci The C-terminal domain of Kv1.3 regulates functional interactions with the KCNE4 subunit. | ||
FASEB J The calmodulin-binding tetraleucine motif of KCNE4 is responsible for association with Kv1.3. |