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KCNH7 Polyclonal antibody

KCNH7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13622-1-AP

Print datasheet

Synonyms

Eag related protein 3, ERG 3, ERG3, hERG 3, HERG3, KCNH7, Kv11.3

验证数据展示

  • WB analysis of human brain using 13622-1-AP

    WB analysis of human brain using 13622-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13622-1-AP (KCNH7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 13622-1-AP

    IHC staining of human heart using 13622-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13622-1-AP (KCNH7 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 13622-1-AP

    IHC staining of human heart using 13622-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13622-1-AP (KCNH7 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of mouse brain using 13622-1-AP

    IP experiment of mouse brain using 13622-1-AP

    IP result of anti-KCNH7 (IP:13622-1-AP, 4ug; Detection:13622-1-AP 1:300) with mouse brain tissue lysate 5200ug.

经过测试的应用

Positive WB detected inhuman brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13622-1-AP targets KCNH7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KCNH7 fusion protein Ag4532 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name potassium voltage-gated channel, subfamily H (eag-related), member 7
Synonyms Eag related protein 3, ERG 3, ERG3, hERG 3, HERG3, KCNH7, Kv11.3
Calculated Molecular Weight 1196 aa, 135 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC035815
Gene Symbol KCNH7
Gene ID (NCBI) 90134
RRIDAB_10638620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NS40
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KCNH7 antibody 13622-1-APDownload protocol
IHC protocol for KCNH7 antibody 13622-1-APDownload protocol
IP protocol for KCNH7 antibody 13622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols