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验证数据展示

  • WB analysis using 83674-1-RR

    WB analysis using 83674-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig brain using 83674-1-RR

    WB analysis of pig brain using 83674-1-RR

    pig brain tissue were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83674-1-RR

    IHC staining of mouse brain using 83674-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83674-1-RR (KCNH7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83674-1-RR

    Affinity and Kinetic Characterization of 83674-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83674-1-RR against Human KCNH7 were performed. The affinity constant is below 1 pM.

KCNH7 Recombinant antibody

KCNH7 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83674-1-RR

产品说明书

CloneNo. 240644B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

ERG-3, ERG3, ERG 3, Eag-related protein 3, Eag related protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, pig brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83674-1-RR targets KCNH7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 KCNH7 fusion protein Ag4532 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 potassium voltage-gated channel, subfamily H (eag-related), member 7
别名 ERG-3, ERG3, ERG 3, Eag-related protein 3, Eag related protein 3
计算分子量 1196 aa, 135 kDa
观测分子量 135 kDa
GenBank蛋白编号BC035815
基因名称 KCNH7
Gene ID (NCBI) 90134
RRIDAB_3671279
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NS40
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Potassium voltage-gated channel subfamily H member 7 is a protein that in humans is encoded by the KCNH7 gene. The protein encoded by this gene is a voltage-gated potassium channel subunit. KCNH7 is pore-forming (alpha) subunit of voltage-gated potassium channel. Channel properties may be modulated by cAMP and subunit assembly.

实验方案

Product Specific Protocols
WB protocol for KCNH7 antibody 83674-1-RRDownload protocol
IHC protocol for KCNH7 antibody 83674-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols