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验证数据展示

  • WB analysis of human cerebellum using 14212-1-AP

    WB analysis of human cerebellum using 14212-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14212-1-AP (KChIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14212-1-AP

    IHC staining of human pancreas cancer using 14212-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas cancer using 14212-1-AP

    IHC staining of human pancreas cancer using 14212-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of fetal human brain using 14212-1-AP

    IP experiment of fetal human brain using 14212-1-AP

    IP result of anti-KChIP1 (IP:14212-1-AP, 4ug; Detection:14212-1-AP 1:300) with fetal human brain tissue lysate 3500 ug.

  • FC experiment of HepG2 using 14212-1-AP

    FC experiment of HepG2 using 14212-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KChIP1 Polyclonal antibody (14212-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

KChIP1 Polyclonal antibody

KChIP1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Cat No. 14212-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), IP, ELISA

别名

KCNIP1, A-type potassium channel modulatory protein 1, Kv channel-interacting protein 1, Potassium channel-interacting protein 1, VABP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman cerebellum tissue, human brain tissue
Positive IP detected infetal human brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14212-1-AP targets KChIP1 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 KChIP1 fusion protein Ag5432 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Kv channel interacting protein 1
别名 KCNIP1, A-type potassium channel modulatory protein 1, Kv channel-interacting protein 1, Potassium channel-interacting protein 1, VABP
计算分子量 227 aa, 27 kDa
观测分子量 25 kDa
GenBank蛋白编号BC050375
基因名称 KCNIP1
Gene ID (NCBI) 30820
RRIDAB_2265412
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZI2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.

实验方案

Product Specific Protocols
WB protocol for KChIP1 antibody 14212-1-APDownload protocol
IHC protocol for KChIP1 antibody 14212-1-APDownload protocol
IP protocol for KChIP1 antibody 14212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols