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验证数据展示

  • WB analysis of mouse brain using 12032-1-AP

    WB analysis of mouse brain using 12032-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12032-1-AP

    IHC staining of human gliomas using 12032-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12032-1-AP (KCNIP3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human gliomas using 12032-1-AP

    IHC staining of human gliomas using 12032-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12032-1-AP (KCNIP3 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse brain using 12032-1-AP

    IP experiment of mouse brain using 12032-1-AP

    IP result of anti-KCNIP3 (IP:12032-1-AP, 4ug; Detection:12032-1-AP 1:1000) with mouse brain tissue lysate 4400 ug.

  • IF Staining of SH-SY5Y using 12032-1-AP

    IF Staining of SH-SY5Y using 12032-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using KCNIP3 antibody (12032-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

KCNIP3 Polyclonal antibody

KCNIP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12032-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Kv channel-interacting protein 3, DRE-antagonist modulator, Calsenilin, A-type potassium channel modulatory protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12032-1-AP targets KCNIP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 KCNIP3 fusion protein Ag2665 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Kv channel interacting protein 3, calsenilin
别名 Kv channel-interacting protein 3, DRE-antagonist modulator, Calsenilin, A-type potassium channel modulatory protein 3
计算分子量 256 aa, 29 kDa
观测分子量 29 kDa, 27 kDa
GenBank蛋白编号BC012850
基因名称 KCNIP3
Gene ID (NCBI) 30818
RRIDAB_2234348
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2W7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KCNIP3 antibody 12032-1-APDownload protocol
IHC protocol for KCNIP3 antibody 12032-1-APDownload protocol
IF protocol for KCNIP3 antibody 12032-1-APDownload protocol
IP protocol for KCNIP3 antibody 12032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols